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Cat.¹: PMQ-007-50-F-CE Name: AmpliSens® Pyroscreen CROHN-screen Number of tests: 55 Detection Method: Pyrosequencing Packaging arrangement (PA): Kit with reagents only for amplification step Content: All reagents are in individual vials (no PCR-tubes) Shelf life: 9 months |
| AmpliSens® Pyroscreen CROHN-screen kit Instruction Manual (version 04.07.2018, update: 23.11.2018) |
AmpliSens® Pyroscreen CROHN-screen kit is an in vitro nucleic acid amplification test based on pyrosequencing technology for genotyping the single-nucleotide polymorphisms (SNPs).
AmpliSens® Pyroscreen CROHN-screen kit is intended for detection of genetic polymorphisms in genes associated with susceptibility to Crohn's disease.
Polymorphisms for analysis
|
Locus |
Product |
Polymorphism |
rs |
Sequence to Analyze |
Analysis |
Genotype variant |
|
NOD2 |
Caspase activator |
R702W C>T |
rs2066844 |
TCC/TGGCGCCAGG |
forward |
CC CT TT |
|
NOD2 |
Caspase activator |
G908R G>C |
rs2066845 |
C/GCCAGAATCTGA |
reverse |
GG GC CC |
|
NKX2-3 |
Transcription factor |
A>G |
rs10883365 |
C/TACCTTCAAACCG |
reverse |
AA AG GG |
|
PTPN2 |
Tyrosine phosphatase |
T>G |
rs2542151 |
CT/GCTTCCTGAG |
forward |
TT TG GG |
Recommended extraction kits: RIBO-prep (K2-9-Et-100-CE), DNA-sorb-B (K1-2-100-CE). It is possible to use other nucleic acid extraction protocols.
Nesessary reagent kit for sample preparation PEERO-prep (K15-1611-40-CE).
NOTICE: This kit is compatible only with PyroMark Gold Q24 or PyroMark Gold Q96 MD instrument (QIAGEN, Germany).
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