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EC CERTIFICATE

EC Declaration of Conformity Annex (List B)

EC Declaration of Conformity Annex (Others)

CERTIFICATE ISO 13485

InterLabService
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AmpliSens
AmpliSens Pyroscreen CROHN-screen
Cat.: PMQ-007-50-F-CE
Name: AmpliSens Pyroscreen CROHN-screen
Number of tests: 55
Detection Method: Pyrosequencing
Packaging arrangement (PA): Kit with reagents only for amplification step
Content: All reagents are in individual vials (no PCR-tubes)
Shelf life: 9 months

AmpliSens Pyroscreen CROHN-screen kit Instruction Manual (version 04.07.2018, update: 23.11.2018)


AmpliSens Pyroscreen CROHN-screen kit is an in vitro nucleic acid amplification test based on pyrosequencing technology for genotyping the single-nucleotide polymorphisms (SNPs).

AmpliSens Pyroscreen CROHN-screen kit is intended for detection of genetic polymorphisms in genes associated with susceptibility to Crohn's disease.


Polymorphisms for analysis

Locus

Product

Polymorphism

rs

Sequence to Analyze

Analysis

Genotype variant

NOD2

Caspase activator

R702W C>T

rs2066844

TCC/TGGCGCCAGG

forward

CC

CT

TT

NOD2

Caspase activator

G908R G>C

rs2066845

C/GCCAGAATCTGA

reverse

GG

GC

CC

NKX2-3

Transcription factor

A>G

rs10883365

C/TACCTTCAAACCG

reverse

AA

AG

GG

PTPN2

Tyrosine phosphatase

T>G

rs2542151

CT/GCTTCCTGAG

forward

TT

TG

GG

Recommended extraction kits:RIBO-prep(K2-9-Et-100-CE),DNA-sorb-B(K1-2-100-CE). It is possible to use other nucleic acid extraction protocols.

Nesessary reagent kit for sample preparationPEERO-prep(K15-1611-40-CE).

NOTICE: This kit is compatible only with PyroMark Gold Q24 or PyroMark Gold Q96 MD instrument (QIAGEN, Germany).


 
Attention!!! Number of tests includes controls and calibrators (for quantitative tests).

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