Quantitation of all Four Gardnerella vaginalis Clades Detects Abnormal Vaginal Microbiota Characteristic of Bacterial Vaginosis More Accurately than Putative G. vaginalis Sialidase A Gene Count
High Gardnerella vaginalis overall load predicts abnormal vaginal microbiota characteristic of bacterial vaginosis (BV) better than high sialidase A gene counts.
Although G. vaginalis clade 4 strains appear to lack the sialidase A gene, it is strongly associated with BV microbiota.
Materials ans Methods
Patients, Specimens and Bacterial Vaginosis Diagnosis
Quantitative Polymerase Chain Reaction (PCR) Detection of the Gardnerella vaginalis Clades and the Putative G. vaginalis Sialidase A Gene
Conflict of interest
The study was approved by the Ethical Committee at the D.O. Ott Research Institute of Obstetrics, Gynaecology and Reproductology, St Petersburg (approval number 73/2015).
Informed consent was obtained from all individual participants included in the study.
1. Koumans EH, Sternberg M, Bruce C, McQuillan G, Kendrick J, Sutton M, et al. The prevalence of bacterial vaginosis in the United States, 2001–2004; associations with symptoms, sexual behaviors, and reproductive health. Sex Transm Dis. 2007;34:864–869. doi: 10.1097/OLQ.0b013e318074e565. [PubMed] [CrossRef] [Google Scholar]